3β hsdh activity Search Results


3β hsdh activity  (ATCC)
98
ATCC 3β hsdh activity
3β Hsdh Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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3β-hsd inhibitor trilostane  (Sanofi)
90
Sanofi 3β-hsd inhibitor trilostane
3β Hsd Inhibitor Trilostane, supplied by Sanofi, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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thermal cycler  (MJ Research)
90
MJ Research thermal cycler
Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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sc 515120 af488  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology sc 515120 af488
Sc 515120 Af488, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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goat anti human 3β hsd antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology goat anti human 3β hsd antibody
Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and <t>3</t> beta-hydroxysteroid dehydrogenase <t>(3β-HSD)</t> are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.
Goat Anti Human 3β Hsd Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti human 3β hsd antibody/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
goat anti human 3β hsd antibody - by Bioz Stars, 2026-03
94/100 stars
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3β hsd1  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology 3β hsd1
Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and <t>3</t> beta-hydroxysteroid dehydrogenase <t>(3β-HSD)</t> are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.
3β Hsd1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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85/100 stars
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3β hsd  (Proteintech)
96
Proteintech 3β hsd
Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and <t>3</t> beta-hydroxysteroid dehydrogenase <t>(3β-HSD)</t> are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.
3β Hsd, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3β hsd/product/Proteintech
Average 96 stars, based on 1 article reviews
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96/100 stars
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3β-hsd activity assay  (RAMSEY MEDICAL INC)
90
RAMSEY MEDICAL INC 3β-hsd activity assay
Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and <t>3</t> beta-hydroxysteroid dehydrogenase <t>(3β-HSD)</t> are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.
3β Hsd Activity Assay, supplied by RAMSEY MEDICAL INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
3β-hsd activity assay - by Bioz Stars, 2026-03
90/100 stars
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3β-hsd activity  (DuPont de Nemours)
90
DuPont de Nemours 3β-hsd activity
Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and <t>3</t> beta-hydroxysteroid dehydrogenase <t>(3β-HSD)</t> are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.
3β Hsd Activity, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
3β-hsd activity - by Bioz Stars, 2026-03
90/100 stars
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3β hsd2 lentiviral activation particles  (Santa Cruz Biotechnology)
N/A
CRISPR Cas9 KO Plasmids consists of 3β HSD2 specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce a site specific
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3β hsd3 crispr activation plasmid  (Santa Cruz Biotechnology)
N/A
Target species mouse CRISPR Cas9 KO Plasmids consists of 3β HSD3 specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce
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3β hsd1 crispr activation plasmid  (Santa Cruz Biotechnology)
N/A
Target species mouse CRISPR Cas9 KO Plasmids consists of 3β hsd1 specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce
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Image Search Results


Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and 3 beta-hydroxysteroid dehydrogenase (3β-HSD) are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Function in Modulating Human Granulosa Cell Steroidogenesis and Female Fertility

doi: 10.3390/ijms21103592

Figure Lengend Snippet: Alteration of key steroidogenic enzymes in various infertile groups. Steroidogenic acute regulatory protein (StAR) and 3 beta-hydroxysteroid dehydrogenase (3β-HSD) are key steroidogenic enzymes. ( A ) Representative immunoblots reveal StAR (30 kDa) and 3β-HSD (43 kDa) levels in granulosa cells, as determined by Western blot; α-tubulin (50 kDa) was used as an internal control. ( B ) Data quantification of StAR and 3β-HSD. ( C ). Linear regression analysis was performed on serum E2 content with StAR or serum E2 content with 3β-HSD in granulosa cells.

Article Snippet: After the transfer was completed, the PVDF membrane was placed in a blocking buffer for 60 min. Then, the primary antibodies, rabbit anti-human StAR antibody (cat# ab3343, Abcam) and goat anti-human 3β-HSD antibody (cat# sc-30821, Santa Cruz), and then the secondary antibody anti-goat IgG-HRP (cat# sc-2020, Santa Cruz), were incubated with the membrane, which was followed by it being treated with enhanced chemiluminescence detection (ECL) using an ECL system (GE Healthcare Life Sciences, Chicago, IL, USA).

Techniques: Western Blot, Control

Mitochondrial function in modulating human granulosa cell steroidogenesis and female fertility. Cholesterol transferred from granulosa cells, by low-density lipoprotein (LDL) receptor-mediated endocytosis, into theca cells, where it is used as a substrate for steroidogenesis. The conversion of cholesterol to pregnenolone is initiated by the binding of luteinizing hormone (LH) to the LH receptor (LHR), and subsequent conversion of androgens to E2 is initiated by the binding of follicle-stimulating hormone (FSH) to the follicle-stimulating hormone receptor (FSHR). Mitochondria are the central sites for steroid hormone biosynthesis. The first step in the biosynthesis of steroid hormones is the transfer of cholesterol to the mitochondrial outer membrane, which is facilitated by StAR. Then, cytochrome P450scc (CYP11A1) initiates steroidogenesis by converting cholesterol to pregnenolone at the mitochondrial inner membrane, and the enzyme 3β-HSD binds with P450scc to form a complex inserted into the mitochondrial inner membrane of the mitochondria to synthesize progesterone. The mitochondrial intermembrane proton gradient is essential for the 3β-HSD activity. Mitochondrial dysfunction of the human granulosa cells may contribute to the decline of steroidogenesis.

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Function in Modulating Human Granulosa Cell Steroidogenesis and Female Fertility

doi: 10.3390/ijms21103592

Figure Lengend Snippet: Mitochondrial function in modulating human granulosa cell steroidogenesis and female fertility. Cholesterol transferred from granulosa cells, by low-density lipoprotein (LDL) receptor-mediated endocytosis, into theca cells, where it is used as a substrate for steroidogenesis. The conversion of cholesterol to pregnenolone is initiated by the binding of luteinizing hormone (LH) to the LH receptor (LHR), and subsequent conversion of androgens to E2 is initiated by the binding of follicle-stimulating hormone (FSH) to the follicle-stimulating hormone receptor (FSHR). Mitochondria are the central sites for steroid hormone biosynthesis. The first step in the biosynthesis of steroid hormones is the transfer of cholesterol to the mitochondrial outer membrane, which is facilitated by StAR. Then, cytochrome P450scc (CYP11A1) initiates steroidogenesis by converting cholesterol to pregnenolone at the mitochondrial inner membrane, and the enzyme 3β-HSD binds with P450scc to form a complex inserted into the mitochondrial inner membrane of the mitochondria to synthesize progesterone. The mitochondrial intermembrane proton gradient is essential for the 3β-HSD activity. Mitochondrial dysfunction of the human granulosa cells may contribute to the decline of steroidogenesis.

Article Snippet: After the transfer was completed, the PVDF membrane was placed in a blocking buffer for 60 min. Then, the primary antibodies, rabbit anti-human StAR antibody (cat# ab3343, Abcam) and goat anti-human 3β-HSD antibody (cat# sc-30821, Santa Cruz), and then the secondary antibody anti-goat IgG-HRP (cat# sc-2020, Santa Cruz), were incubated with the membrane, which was followed by it being treated with enhanced chemiluminescence detection (ECL) using an ECL system (GE Healthcare Life Sciences, Chicago, IL, USA).

Techniques: Binding Assay, Membrane, Activity Assay